| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |

Western blot analysis of P glycoprotein/ABCB1 using anti-P glycoprotein/ABCB1 antibody (BM0508). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human DLD1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-P glycoprotein/ABCB1 antigen affinity purified monoclonal antibody (BM0508) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P glycoprotein/ABCB1 at approximately 200 kDa. The expected band size for P glycoprotein/ABCB1 is at 141 kDa.

Western blot analysis of P glycoprotein/ABCB1 using anti-P glycoprotein/ABCB1 antibody (BM0508). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human DLD1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-P glycoprotein/ABCB1 antigen affinity purified monoclonal antibody (BM0508) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P glycoprotein/ABCB1 at approximately 200 kDa. The expected band size for P glycoprotein/ABCB1 is at 141 kDa.




