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Anti-Gamma Tubulin/TUBG1 Antibody (Clone#GTU-88)

Mouse Monoclonal Antibody

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筛选器: All WB IHC

BM1606

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti- γ-Tubulin antibody (BM1606). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: Hela whole cell lysates,
    Lane 2: COLO-320 whole cell lysates,
    Lane 3: HepG2 whole cell lysates,
    Lane 4: U-87MG whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat C6 whole cell lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse HEPA1-6 whole cell lysates.
    Use mouse anti- γ-Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for γ-Tubulin at approximately 55KD. The expected band size for γ-Tubulin is at 55KD.

    all(3)
  • IHC analysis using anti- γ-Tubulin antibody (BM1606). detected in paraffin-embedded section of rat intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(3)
  • IHC analysis using anti- γ-Tubulin antibody (BM1606). detected in paraffin-embedded section of mouse intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(3)

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产品名称
Anti-Gamma Tubulin/TUBG1 Antibody (Clone#GTU-88)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Gamma 1 tubulin; gamma Tubulin; GCP 1; TUBG; TUBG1; TUBGCP1; Tubulin gamma 1 chain; tubulin; gamma 1
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
TUBG1
克隆号
GTU-88
宿主
Mouse
抗体亚型
IgG1
免疫原
Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.
计算分子量
51 kDa
实际分子量
55 kDa
成分
200 ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
Uniprot ID
P23258  
文献引用格式
Gamma Tubulin/TUBG1 Antibody (Clone#GTU-88) (Boster Biological Technology, Wuhan, China. Catalog#BM1606)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Gamma Tubulin/TUBG1 Antibody (Clone#GTU-88)

    筛选器: All WB IHC

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