Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of anti- γ-Tubulin antibody (BM1606). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: COLO-320 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: U-87MG whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
Use mouse anti- γ-Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for γ-Tubulin at approximately 55KD. The expected band size for γ-Tubulin is at 55KD.
IHC analysis using anti- γ-Tubulin antibody (BM1606). detected in paraffin-embedded section of rat intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IHC analysis using anti- γ-Tubulin antibody (BM1606). detected in paraffin-embedded section of mouse intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Western blot analysis of anti- γ-Tubulin antibody (BM1606). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: COLO-320 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: U-87MG whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
Use mouse anti- γ-Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for γ-Tubulin at approximately 55KD. The expected band size for γ-Tubulin is at 55KD.
IHC analysis using anti- γ-Tubulin antibody (BM1606). detected in paraffin-embedded section of rat intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IHC analysis using anti- γ-Tubulin antibody (BM1606). detected in paraffin-embedded section of mouse intestinal tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.