Anti-ERBB4 Antibody (Clone#HC-5)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP FCM

BM3935

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ERBB4 antibody (BM3935). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERBB4 antigen affinity purified monoclonal antibody (BM3935) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERBB4 at approximately 180 kDa. The expected band size for ERBB4 is at 147 kDa.
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Immunofluorescent analysis of A673 cells, using ErbB4 (HER4) Antibody .
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产品简介>

产品名称

Anti-ERBB4 Antibody (Clone#HC-5)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

4ICD; E4ICD; ERBB4; HER4; p180erbB4; s80HER4

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IP, FCM

基因名称

ERBB4

克隆号

HC-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ErbB (HER4)

计算分子量

147 kDa

实际分子量

180 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ERBB4(V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4) also known as ONCOGENE ERBB4 or HER4, is an enzyme that in humans is encoded by the ERBB4 gene. The HER4/ERBB4 gene is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. The ERBB4 gene is mapped on 2q34. ERBB4 is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domainbinding motif. Huang et al.(2000) reported that ERBB4 is enriched in the postsynaptic density and associates with PSD95. ERBB4 is a transmembrane receptor tyrosine kinase that regulates cell proliferation and differentiation. After binding its ligand, heregulin, or activation of protein kinase C by TPA, the ERBB4 ectodomain is cleaved by a metalloprotease. Ni et al.(2001)concluded that gamma-secretase cleavage of ERBB4 may represent another mechanism for receptor tyrosine kinase-mediated signaling.

Uniprot ID

Q15303

文献引用格式

ERBB4 Antibody (Clone#HC-5) (Boster Biological Technology, Wuhan, China. Catalog#BM3935)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Receptor tyrosine-protein kinase erbB-4

实验方案>

实验步骤

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