Figure 1. Western blot analysis of anti-HMGB1 antibody (BM3965). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified monoclonal antibody (BM3965) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
Figure 2. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 3. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 4. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 5. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 6. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 7. IF analysis of HMGB1 using anti-HMGB1 antibody (BM3965) and anti-Beta Tubulin antibody (M01857-3).
HMGB1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| Flow Cytometry (FCM): | 1:30 |
Figure 1. Western blot analysis of anti-HMGB1 antibody (BM3965). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified monoclonal antibody (BM3965) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
Figure 2. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 3. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 4. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 5. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 6. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 7. IF analysis of HMGB1 using anti-HMGB1 antibody (BM3965) and anti-Beta Tubulin antibody (M01857-3).
HMGB1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
Figure 1. Western blot analysis of anti-HMGB1 antibody (BM3965). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified monoclonal antibody (BM3965) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
Figure 2. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 3. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 4. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 5. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 6. IHC analysis of HMGB1 using anti-HMGB1 antibody (BM3965) .
HMGB1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Figure 7. IF analysis of HMGB1 using anti-HMGB1 antibody (BM3965) and anti-Beta Tubulin antibody (M01857-3).
HMGB1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-HMGB1 Antibody (BM3965) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
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