Anti-Cytokeratin 20/KRT20 Antibody (Clone#CDI-11)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP FCM

BM4207

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-Cytokeratin 20/KRT20 antibody (BM4207). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 20/KRT20 antigen affinity purified monoclonal antibody (BM4207) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 20/KRT20 at approximately 48 kDa. The expected band size for Cytokeratin 20/KRT20 is at 48 kDa.
all(7)
IHC analysis of Cytokeratin 20/KRT20 using anti-Cytokeratin 20/KRT20 antibody (BM4207) .
Cytokeratin 20/KRT20 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 20/KRT20 Antibody (BM4207) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of Cytokeratin 20/KRT20 using anti-Cytokeratin 20/KRT20 antibody (BM4207) .
Cytokeratin 20/KRT20 was detected in a paraffin-embedded section of human oppendix tissue. The tissue section was incubated with rabbit anti-Cytokeratin 20/KRT20 Antibody (BM4207) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
Immunofluorescent analysis using the Antibody.
all(7)
Immunofluorescent analysis of Hela cells, using Cytokeratin 20 Antibody .
all(7)
all(7)
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Cytokeratin 20/KRT20 Antibody (Clone#CDI-11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; CK 20; CK20; Cytokeratin 20; Cytoskeleton Marker; K20; keratin 20; KRT20; KRT21; Protein IT

产品类型

Monoclonal

检验物种

human, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

KRT20

克隆号

CDI-11

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Cytokeratin 20

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine.

Uniprot ID

P35900

文献引用格式

Cytokeratin 20/KRT20 Antibody (Clone#CDI-11) (Boster Biological Technology, Wuhan, China. Catalog#BM4207)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Keratin, type I cytoskeletal 20

实验方案>

实验步骤

引用文献(0)>

清除