Anti-Calreticulin/CALR Antibody (Clone#CGO-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4228

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Calreticulin/CALR antibody (BM4228). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Colo320 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calreticulin/CALR antigen affinity purified monoclonal antibody (BM4228) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calreticulin/CALR at approximately 60 kDa. The expected band size for Calreticulin/CALR is at 48 kDa.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human renal cancer, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human placenta, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse testis, using the Antibody at 1:150 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse cerebellum, using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Calreticulin/CALR Antibody (Clone#CGO-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CALR; Calregulin; calreticulin; cC1qR; CRP55; CRT; CRTC; ERp60; grp60; HACBP; RO; SSA

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

CALR

克隆号

CGO-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Calreticulin - ER Marker

计算分子量

48 kDa

实际分子量

50-60 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Calreticulin also known as°Calregulin,°CRP55,°CaBP3,°Calsequestrin-like protein, and endoplasmic reticulum resident protein 60 (ERp60) is a protein that in humans is encoded by the°CALR gene. It is mapped to 19p13.13. Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Uniprot ID

P27797

文献引用格式

Calreticulin/CALR Antibody (Clone#CGO-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4228)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Calreticulin

实验方案>

实验步骤

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