Anti-RUNX1/RUNX2/RUNX3 Antibody (Clone#DOG-18)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP FCM

BM4265

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-RUNX1/RUNX2/RUNX3 antibody (BM4265). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RUNX1/RUNX2/RUNX3 antigen affinity purified monoclonal antibody (BM4265) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RUNX1/RUNX2/RUNX3 at approximately 51 kDa. The expected band size for RUNX1/RUNX2/RUNX3 is at 51 kDa.
all(5)
Immunohistochemical analysis of paraffin-embedded mouse lung, using RUNX1/RUNX2/RUNX3 Antibody.
all(5)
all(5)
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-RUNX1/RUNX2/RUNX3 Antibody (Clone#DOG-18)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

RUNX1; AML1; RUNX2; OSF2; RUNX3; CBFA3

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

RUNX1/RUNX2/RUNX3

克隆号

DOG-18

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human RUNX1/RUNX2/RUNX3

实际分子量

51 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Runt-related transcription factor 1 (RUNX1), also known as AML1 or CBFA2, is a protein that in humans is encoded by the RUNX1 gene. It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). RUNX1 is mapped to 21q22.12. RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. Chromosomal translocations involving the RUNX1 gene are associated with several types of leukemia including M2 AML. Mutations in RUNX1 are implicated in cases of breast cancer.

Uniprot ID

Q01196/Q13761/Q13950

文献引用格式

RUNX1/RUNX2/RUNX3 Antibody (Clone#DOG-18) (Boster Biological Technology, Wuhan, China. Catalog#BM4265)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Runt-related transcription factor 1

实验方案>

实验步骤

引用文献(0)>

清除