Anti-HSP60/HSPD1 Antibody (Clone#DOI-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM4267

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of Hsp60 expression in (1) HeLa cell lysate; (2) 293T cell lysate; (3) NIH/3T3 cell lysate; (4) Mouse heart lysate; (5) PC-12 cell lysate; (6) Rat heart lysate.
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Hsp60 Antibody .
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产品简介>

产品名称

Anti-HSP60/HSPD1 Antibody (Clone#DOI-8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HSP 60; HSP60; HSP65; HSPD1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

HSPD1

克隆号

DOI-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Hsp60

计算分子量

61 kDa

实际分子量

61 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Uniprot ID

P10809

文献引用格式

HSP60/HSPD1 Antibody (Clone#DOI-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4267)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

60 kDa heat shock protein, mitochondrial

实验方案>

实验步骤

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