Anti-Ubiquitin/UBB Antibody (Clone#DIG-21)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4359

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Ubiquitin/UBB antibody (BM4359). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Ubiquitin/UBB antigen affinity purified monoclonal antibody (BM4359) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Ubiquitin/UBB at approximately 10 kDa. The expected band size for Ubiquitin/UBB is at 26 kDa.
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Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded human colon, using Ubiquitin Antibody.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody.
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Immunofluorescent analysis of Raji cells, using Ubiquitin Antibody .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Ubiquitin/UBB Antibody (Clone#DIG-21)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Polyubiquitin B; UBB; ubiquitin; ubiquitin B

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

UBB

克隆号

DIG-21

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Ubiquitin

计算分子量

26 kDa

实际分子量

10 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Ubiquitin (originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Uniprot ID

P0CG47

文献引用格式

Ubiquitin/UBB Antibody (Clone#DIG-21) (Boster Biological Technology, Wuhan, China. Catalog#BM4359)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Polyubiquitin-B

实验方案>

实验步骤

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