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Anti-JNK1/2/3 (Phospho-T183+T183+T221) Antibody (Clone#EAG-13)

Rabbit Monoclonal antibody

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筛选器: All WB IHC ICC/IF IP FCM

BM4380

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of JNK1/2/3 phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.

    all(11)
  • All lanes use the Antibody for 1 hour at room temperature.

    all(11)
  • IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
    P-JNK1/2/3 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
    P-JNK1/2/3 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
    P-JNK1/2/3 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
    P-JNK1/2/3 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • Immunofluorescent analysis using the Antibody.

    all(11)
  • Immunofluorescent analysis using the Antibody.

    all(11)
  • Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.

    all(11)

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产品简介>

产品名称
Anti-JNK1/2/3 (Phospho-T183+T183+T221) Antibody (Clone#EAG-13)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
c Jun N terminal kinase 1; JNK 46; JNK1; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; MAPK8; PRKM8; SAPK1; c Jun N terminal kinase 2; JNK 55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAP kinase 9; MAPK 9; MAPK9; p54a; p54aSAPK; PRKM9; SAPK; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; SAPK1b; c-Jun N-terminal kinase 3; MAPK10; JNK3; JNK3A; PRKM10; p493F12; p54bSAPK
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, IP, FCM
基因名称
MAPK8/MAPK9/MAPK10
克隆号
EAG-13
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human Phospho-JNK1/2/3 (T183+T183+T221) Phospho-JNK1/2/3 (T183+T183+T221)
计算分子量
48 kDa
实际分子量
48 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Uniprot ID
文献引用格式
JNK1/2/3 (Phospho-T183+T183+T221) Antibody (Clone#EAG-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4380)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:50
Flow Cytometry (FCM):1:50

实验方案>

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    Anti-JNK1/2/3 (Phospho-T183+T183+T221) Antibody (Clone#EAG-13)

    筛选器: All WB IHC ICC/IF IP FCM

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