Anti-KRAS/HRAS/NRAS Antibody (Clone#EBE-14)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF IP FCM

BM4388

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-KRAS/HRAS/NRAS antibody (BM4388). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KRAS/HRAS/NRAS antigen affinity purified monoclonal antibody (BM4388) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for KRAS/HRAS/NRAS at approximately 21 kDa. The expected band size for KRAS/HRAS/NRAS is at 21 kDa.
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产品简介>

产品名称

Anti-KRAS/HRAS/NRAS Antibody (Clone#EBE-14)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

GTPase NRas; Transforming protein N-Ras; NRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; HRAS; HRAS1; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; KRAS; HRAS; KRAS2; RASK2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, IP, FCM

基因名称

KRAS/HRAS/NRAS

克隆号

EBE-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human KRAS+HRAS+NRAS

计算分子量

21 kDa

实际分子量

21 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot ID

P01111/P01112/P01116

文献引用格式

KRAS/HRAS/NRAS Antibody (Clone#EBE-14) (Boster Biological Technology, Wuhan, China. Catalog#BM4388)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

GTPase NRas

实验方案>

实验步骤

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