Anti-Cytokeratin 10/KRT10 Antibody (Clone#EEF-11)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4422

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Cytokeratin 10/KRT10 antibody (BM4422). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat skin tissue lysates,
Lane 4: rat stomach tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 10/KRT10 antigen affinity purified monoclonal antibody (BM4422) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 10/KRT10 at approximately 59 kDa. The expected band size for Cytokeratin 10/KRT10 is at 59 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder, using Cytokeratin 10 Antibody.
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Immunofluorescent analysis of HACAT cells, using Cytokeratin 10 Antibody.
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产品简介>

产品名称

Anti-Cytokeratin 10/KRT10 Antibody (Clone#EEF-11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; CK 10; CK10; Cytokeratin 10; Cytoskeleton Marker; K10; keratin 10; KPP; KRT10

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

KRT10

克隆号

EEF-11

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Cytokeratin 10

计算分子量

59 kDa

实际分子量

59 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

Uniprot ID

P13645

文献引用格式

Cytokeratin 10/KRT10 Antibody (Clone#EEF-11) (Boster Biological Technology, Wuhan, China. Catalog#BM4422)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Keratin, type I cytoskeletal 10

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实验步骤

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