Anti-Nestin/NES Antibody (Clone#FBH-14)

Rabbit monoclonal antibody

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筛选器： All WB IHC IF

BM4494

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Nestin/NES antigen affinity purified monoclonal antibody (BM4494) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Nestin/NES at approximately 270-300 kDa. The expected band size for Nestin/NES is at 177 kDa.
all(8)
IHC analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of human Diffuse large B-cell lymphoma of the intestine tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IF analysis of Nestin/NES using anti-Nestin/NES antibody (BM4494).
Nestin/NES was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Nestin/NES Antibody (BM4494) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody.
all(8)

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产品简介>

产品名称

Anti-Nestin/NES Antibody (Clone#FBH-14)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NES; Nestin

产品类型

Monoclonal

检验物种

human, mouse, rat, pig

应用范围

WB, IHC, IF

基因名称

NES

克隆号

FBH-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Nestin

计算分子量

177 kDa

实际分子量

270-300 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins. It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration. Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins. Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells. Expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and is generally accepted as a marker for neural cancer stem cells. Nestin expression has alos been observed in astrocytes, retina, cardiac muscle, pancreas and other tissues. Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

Uniprot ID

P48681

文献引用格式

Nestin/NES Antibody (Clone#FBH-14) (Boster Biological Technology, Wuhan, China. Catalog#BM4494)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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