Anti-Cytokeratin 14/KRT14 Antibody (Clone#FEF-11)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4522

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Cytokeratin 14/KRT14 antibody (BM4522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 14/KRT14 antigen affinity purified monoclonal antibody (BM4522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 14/KRT14 at approximately 52 kDa. The expected band size for Cytokeratin 14/KRT14 is at 52 kDa.
all(3)
Immunohistochemical analysis of paraffin-embedded human stomach, using Cytokeratin 14 Antibody.
all(3)
Immunofluorescent analysis of HACAT cells, using Cytokeratin 14 Antibody.
all(3)

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产品名称

Anti-Cytokeratin 14/KRT14 Antibody (Clone#FEF-11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; CK 14; CK14; Cytokeratin 14; Cytoskeleton Marker; EBS3; EBS4; K14; keratin 14; KRT14; NFJ

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

KRT14

克隆号

FEF-11

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Cytokeratin 14

计算分子量

52 kDa

实际分子量

52 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans it is encoded by the KRT14 gene. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Uniprot ID

P02533

文献引用格式

Cytokeratin 14/KRT14 Antibody (Clone#FEF-11) (Boster Biological Technology, Wuhan, China. Catalog#BM4522)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Keratin, type I cytoskeletal 14

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实验步骤

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