Anti-TWEAKR/TNFRSF12A Antibody (Clone#GFF-20)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

BM4635

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-TWEAKR/TNFRSF12A antibody (BM4635). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HUVEC whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: mouse 4T1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TWEAKR/TNFRSF12A antigen affinity purified monoclonal antibody (BM4635) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TWEAKR/TNFRSF12A at approximately 17,20 kDa. The expected band size for TWEAKR/TNFRSF12A is at 17,20 kDa.
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产品名称

Anti-TWEAKR/TNFRSF12A Antibody (Clone#GFF-20)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

TNF receptor superfamily member 12A; CD266; FN14; TWEAKR; TNFRSF12A

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

TNFRSF12A

克隆号

GFF-20

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human TWEAKR

计算分子量

17,20 kDa

实际分子量

17,20 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 16p13.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

Uniprot ID

Q9NP84

文献引用格式

TWEAKR/TNFRSF12A Antibody (Clone#GFF-20) (Boster Biological Technology, Wuhan, China. Catalog#BM4635)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Tumor necrosis factor receptor superfamily member 12A

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