Anti-Histone H3 Antibody (Clone#HDE-8)

Rabbit Monoclonal Antibody

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筛选器： All WB IHC ICC/IF

BM4715

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Histone H3 antibody (BM4715). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Histone H3 antigen affinity purified monoclonal antibody (BM4715) at a dilution of 1:2000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Histone H3 at approximately 15 kDa. The expected band size for Histone H3 is at 15 kDa.
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Immunohistochemical analysis of paraffin-embedded Rat thymus, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using Histone H3 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human tonsil, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody.
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Immunofluorescent analysis of Hela cells, using Histone H3 Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Histone H3 Antibody (Clone#HDE-8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

H3/b; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone cluster 1; H3d; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

H3C1/H3C2/H3C3/H3C4/H3C6/H3C7/H3C8/H3C10/H3C11/H3C12

克隆号

HDE-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Histone H3

计算分子量

15 kDa

实际分子量

15-17 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Uniprot ID

P68431

文献引用格式

Histone H3 Antibody (Clone#HDE-8) (Boster Biological Technology, Wuhan, China. Catalog#BM4715)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Histone H3.1

实验方案>

实验步骤

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