Anti-E2F1 Antibody (Clone#IGD-5)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4848

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of E2F1 using anti-E2F1 antibody (BM4848). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-E2F1 antigen affinity purified monoclonal antibody (BM4848) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for E2F1 at approximately 65-70 kDa. The expected band size for E2F1 is at 47 kDa.
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IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human lungcancer tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human testis cancer tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(16)
IHC analysis of E2F1 using anti-E2F1 antibody (BM4848).
E2F1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-E2F1 Antibody (BM4848) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HeLa cells, using E2F1 Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-E2F1 Antibody (Clone#IGD-5)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

E2F 1; E2F transcription factor 1; E2F1; PBR3; PRB binding protein E2F 1; RBAP 1; RBAP1; RBBP 3; RBBP3; RBP3; Transcription factor E2F1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

E2F1

克隆号

IGD-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human E2F1

计算分子量

47 kDa

实际分子量

65-70 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product(pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein(pRB).

Uniprot ID

Q01094

文献引用格式

E2F1 Antibody (Clone#IGD-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4848)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Transcription factor E2F1

实验方案>

实验步骤

引用文献(0)>

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