Anti-ELA2/ELANE Antibody (Clone#AOCI-5)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4914

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-ELA2/ELANE antibody (BM4914). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELA2/ELANE antigen affinity purified monoclonal antibody (BM4914) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ELA2/ELANE at approximately 35 kDa. The expected band size for ELA2/ELANE is at 29 kDa.
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产品名称

Anti-ELA2/ELANE Antibody (Clone#AOCI-5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Bone marrow serine protease; ELA2; ELANE; Elastase 2; elastase 2; neutrophil; GE; HLE; HNE; Human leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ELANE

克隆号

AOCI-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Neutrophil Elastase

计算分子量

29 kDa

实际分子量

29 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Uniprot ID

P08246

文献引用格式

ELA2/ELANE Antibody (Clone#AOCI-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4914)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Neutrophil elastase

实验方案>

实验步骤

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