Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Flow Cytometry (FCM): | 1:20 |
Western blot analysis of IFNG using anti-IFNG antibody (BM4939).
Lane 1: recombinant human IFNG protein.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IFNG antigen affinity purified monoclonal antibody (BM4939) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IFNG at approximately 17 kDa.
Western blot analysis of IFNG using anti-IFNG antibody (BM4939).
Lane 1: recombinant human IFNG protein.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IFNG antigen affinity purified monoclonal antibody (BM4939) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IFNG at approximately 17 kDa.