Anti-FAP Antibody (Clone#AbF22)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM5121

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-FAP antibody (BM5121). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAP antigen affinity purified monoclonal antibody (BM5121) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAP at approximately 95 kDa. The expected band size for FAP is at 88 kDa.
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产品名称

Anti-FAP Antibody (Clone#AbF22)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

FAP; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; SIMP

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC

基因名称

FAP

克隆号

AbF22

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human FAP1

计算分子量

88 kDa

实际分子量

97 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

Uniprot ID

Q12884

文献引用格式

FAP Antibody (Clone#AbF22) (Boster Biological Technology, Wuhan, China. Catalog#BM5121)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Prolyl endopeptidase FAP

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实验步骤

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