| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:20 |

Western blot analysis of anti-TGM2 antibody (BM5139). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGM2 antigen affinity purified monoclonal antibody (BM5139) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGM2 at approximately 77 kDa. The expected band size for TGM2 is at 77 kDa.

Western blot analysis of anti-TGM2 antibody (BM5139). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGM2 antigen affinity purified monoclonal antibody (BM5139) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGM2 at approximately 77 kDa. The expected band size for TGM2 is at 77 kDa.




