| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:50 |

Western blot analysis of anti-ITPR1 antibody (BM5235). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ITPR1 antigen affinity purified monoclonal antibody (BM5235) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ITPR1 at approximately 314 kDa. The expected band size for ITPR1 is at 314 kDa.

Western blot analysis of anti-ITPR1 antibody (BM5235). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ITPR1 antigen affinity purified monoclonal antibody (BM5235) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ITPR1 at approximately 314 kDa. The expected band size for ITPR1 is at 314 kDa.




