Anti-PRDX3 Antibody (Clone#ACEG-16)

Rabbit monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP FCM

BM5274

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-PRDX3 antibody (BM5274). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human U251 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDX3 antigen affinity purified monoclonal antibody (BM5274) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRDX3 at approximately 24 kDa. The expected band size for PRDX3 is at 28 kDa.
all(5)
all(5)
all(5)
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PRDX3 Antibody (Clone#ACEG-16)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Antioxidant protein 1; AOP 1; AOP1; HBC189; MER5; Peroxiredoxin 3; Peroxiredoxin III; PRDX3; PRO1748; Protein MER5 homolog; Prx III; PRX3; SP 22

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

PRDX3

克隆号

ACEG-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Peroxiredoxin 3 Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

计算分子量

28 kDa

实际分子量

26 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PRDX3(peroxiredoxin 3) also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human AOP1 protein shares 86% amino acid sequence similarity with mouse Aop1, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.

Uniprot ID

P30048

文献引用格式

PRDX3 Antibody (Clone#ACEG-16) (Boster Biological Technology, Wuhan, China. Catalog#BM5274)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除