Anti-Lamin B2/LMNB2 Antibody (Clone#ADBF-12)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM5380

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Lamin B2/LMNB2 antibody (BM5380). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin B2/LMNB2 antigen affinity purified monoclonal antibody (BM5380) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin B2/LMNB2 at approximately 72 kDa. The expected band size for Lamin B2/LMNB2 is at 70 kDa.
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IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (BM5380) .
Lamin B2/LMNB2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Lamin B2/LMNB2 Antibody (BM5380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-Lamin B2/LMNB2 Antibody (Clone#ADBF-12)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

LAMB2; lamin B2; LMN2; LMNB2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

LMNB2

克隆号

ADBF-12

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Lamin B2 Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

计算分子量

70 kDa

实际分子量

70 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Lamin B2, also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. The LMNB2 gene contains 12 exons. Model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2, is generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.

Uniprot ID

Q03252

文献引用格式

Lamin B2/LMNB2 Antibody (Clone#ADBF-12) (Boster Biological Technology, Wuhan, China. Catalog#BM5380)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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