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Anti-BDKRB2 Antibody (Clone#AFAG-2)

Rabbit monoclonal antibody

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筛选器: All WB IHC IP

BM5667

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Western blot analysis of anti-BDKRB2 antibody (BM5667). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human MCF-7 whole cell lysates,
    Lane 4: human K562 whole cell lysates,
    Lane 5: mouse lung tissue lysates,
    Lane 6: mouse kidney tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified monoclonal antibody (BM5667) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDKRB2 at approximately 78 kDa. The expected band size for BDKRB2 is at 44 kDa.

    all(4)
  • IHC analysis of BDKRB2 using anti-BDKRB2 antibody (BM5667) .
    BDKRB2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-BDKRB2 Antibody (BM5667) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(4)
  • IHC analysis of BDKRB2 using anti-BDKRB2 antibody (BM5667) .
    BDKRB2 was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-BDKRB2 Antibody (BM5667) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(4)

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产品名称
Anti-BDKRB2 Antibody (Clone#AFAG-2)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
bradykinin receptor B2; B2R; BK-2; BK2; BKR2; BRB2; BDKRB2
产品类型
Monoclonal
检验物种
human, mouse
应用范围
WB, IHC, IP
基因名称
BDKRB2
克隆号
AFAG-2
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human BDKRB2 Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
计算分子量
44 kDa
实际分子量
78 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Bradykinin receptor B2 is a G-protein coupled receptor forbradykinin, encoded by the BDKRB2 gene in humans. This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein.
Uniprot ID
P30411  
文献引用格式
BDKRB2 Antibody (Clone#AFAG-2) (Boster Biological Technology, Wuhan, China. Catalog#BM5667)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
ImmunoPrecipitation (IP):1:50

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    Anti-BDKRB2 Antibody (Clone#AFAG-2)

    筛选器: All WB IHC IP

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