Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:50 |
Western blot analysis of anti-BDKRB2 antibody (BM5667). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified monoclonal antibody (BM5667) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDKRB2 at approximately 78 kDa. The expected band size for BDKRB2 is at 44 kDa.
IHC analysis of BDKRB2 using anti-BDKRB2 antibody (BM5667) .
BDKRB2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-BDKRB2 Antibody (BM5667) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BDKRB2 using anti-BDKRB2 antibody (BM5667) .
BDKRB2 was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-BDKRB2 Antibody (BM5667) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of anti-BDKRB2 antibody (BM5667). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified monoclonal antibody (BM5667) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDKRB2 at approximately 78 kDa. The expected band size for BDKRB2 is at 44 kDa.
IHC analysis of BDKRB2 using anti-BDKRB2 antibody (BM5667) .
BDKRB2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-BDKRB2 Antibody (BM5667) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of BDKRB2 using anti-BDKRB2 antibody (BM5667) .
BDKRB2 was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-BDKRB2 Antibody (BM5667) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.