Western blot (WB): | 1:500-2000 |
Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB1102). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (PB1102) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDKRB2 at approximately 50 kDa. The expected band size for BDKRB2 is at 44 kDa.
Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB1102). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (PB1102) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDKRB2 at approximately 50 kDa. The expected band size for BDKRB2 is at 44 kDa.