| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| Flow Cytometry (FCM): | 1:50 |

Western blot analysis of anti-CXCR5 antibody (BM5719). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CXCR5 antigen affinity purified monoclonal antibody (BM5719) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CXCR5 at approximately 42 kDa. The expected band size for CXCR5 is at 42 kDa.

IHC analysis of CXCR5 using anti-CXCR5 antibody (BM5719) .
CXCR5 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-CXCR5 Antibody (BM5719) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of anti-CXCR5 antibody (BM5719). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CXCR5 antigen affinity purified monoclonal antibody (BM5719) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CXCR5 at approximately 42 kDa. The expected band size for CXCR5 is at 42 kDa.

IHC analysis of CXCR5 using anti-CXCR5 antibody (BM5719) .
CXCR5 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-CXCR5 Antibody (BM5719) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.





