Anti-IBA1/AIF1 Antibody (Clone#AFGH-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM5765

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IBA1/AIF1 using anti-IBA1/AIF1 antibody (BM5765). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U937 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IBA1/AIF1 antigen affinity purified monoclonal antibody (BM5765) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IBA1/AIF1 at approximately 17 kDa. The expected band size for IBA1/AIF1 is at 17 kDa.
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IHC analysis of IBA1/AIF1 using anti-IBA1/AIF1 antibody (BM5765).
IBA1/AIF1 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-IBA1/AIF1 Antibody (BM5765) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-IBA1/AIF1 Antibody (Clone#AFGH-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AIF 1; AIF1; G1; IBA1; IBA 1; IBA-1; IRT 1; Protein G1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

AIF1

克隆号

AFGH-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human IBA1

计算分子量

17 kDa

实际分子量

17 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Allograft inflammatory factor 1 (AIF-1), also known as ionized calcium-binding adapter molecule 1(IBA1), is a protein that in humans is encoded by the AIF1 gene. This gene encodes a protein that binds actin and calcium. And this gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.

Uniprot ID

P55008

文献引用格式

IBA1/AIF1 Antibody (Clone#AFGH-1) (Boster Biological Technology, Wuhan, China. Catalog#BM5765)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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