产品名称
Human FLT3LG ELISA Kit
指标别名
Fms related receptor tyrosine kinase 3 ligand; FL; FLG3L; FLT3L; FLT3LG
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
fms-related tyrosine kinase 3 ligand
文献引用格式
Human FLT3LG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0354)
背景资料
Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0354-CAP | 96-well plate precoated with anti- human Flt-3ligand antibody | 1 |
| EK0354-ST | Lyophilized recombinant human Flt-3ligand standard | 10ng/tube×2 |
| EK0354-DA | Biotinylated anti- human Flt-3ligand antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 37 | 186 | 439 | 38 | 191 | 431 |
| Standard deviation | 2.81 | 11.16 | 34.24 | 3.45 | 13.75 | 38.35 |
| CV(%) | 7.6 | 6 | 7.8 | 9.1 | 7.2 | 8.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.041 | 0.111 | 0.187 | 0.367 | 0.672 | 1.066 | 1.665 | 2.033 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)