产品名称
Rat FLT3LG ELISA Kit
指标别名
Fms related receptor tyrosine kinase 3 ligand; FL; FLG3L; FLT3L; FLT3LG
免疫原
Expression system for standard: NS0; Immunogen sequence: G27-R188
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
fms-related tyrosine kinase 3 ligand
文献引用格式
Rat FLT3LG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2101)
背景资料
Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.
试剂盒内容
| Catalog number | Description | Quantity |
| EK2101-CAP | 96-well plate precoated with anti- rat Flt-3 ligand antibody | 1 |
| EK2101-ST | Lyophilized recombinant rat Flt-3 ligand standard | 10ng/tube×2 |
| EK2101-DA | Biotinylated anti- rat Flt-3 ligand antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 160 | 699 | 41 | 184 | 759 |
| Standard deviation | 1.62 | 6.88 | 28.66 | 2.46 | 10.67 | 49.34 |
| CV(%) | 4.5% | 4.3% | 4.1% | 6.0% | 5.8% | 6.5% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.015 | 0.076 | 0.134 | 0.239 | 0.419 | 0.824 | 1.457 | 2.565 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum