产品名称
Human IGFBP2 ELISA Kit
指标别名
BP2; IBP 2; IBP2; IGF binding protein 2; IGF BP53; IGFBP 2; IGFBP2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
insulin-like growth factor binding protein 2, 36kDa
文献引用格式
Human IGFBP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0384)
背景资料
Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0384-CAP | 96-well plate precoated with anti- human IGFBP2 antibody | 1 |
EK0384-ST | Lyophilized recombinant human IGFBP2 standard | 10ng/tubex2 |
EK0384-DA | Biotinylated anti- human IGFBP2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 128 | 598 | 1114 | 133 | 553 | 1221 |
Standard deviation | 5.88 | 47.24 | 61.27 | 7.84 | 54.19 | 83.02 |
CV(%) | 4.6 | 7.9 | 5.5 | 5.9 | 9.8 | 6.8 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.137 | 0.218 | 0.283 | 0.422 | 0.691 | 1.201 | 1.916 | 2.423 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine