产品名称
Mouse IGFBP2 ELISA Kit
指标别名
BP2; IBP 2; IBP2; IGF binding protein 2; IGF BP53; IGFBP 2; IGFBP2
免疫原
Expression system for standard: NS0; Immunogen sequence: E35-Q305
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Insulin-like growth factor-binding protein 2
文献引用格式
Mouse IGFBP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0385)
背景资料
Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0385-CAP | 96-well plate precoated with anti- mouse IGFBP2 antibody | 1 |
EK0385-ST | Lyophilized recombinant mouse IGFBP2 standard | 10ng/tubex2 |
EK0385-DA | Biotinylated anti- mouse IGFBP2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 160 | 970 | 3281 | 169 | 968 | 3467 |
Standard deviation | 7.52 | 76.63 | 203.42 | 8.95 | 86.15 | 256.55 |
CV(%) | 4.7% | 7.9% | 6.2% | 5.3% | 8.9% | 7.4% |
典型数据示例
Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
O.D. | 0.088 | 0.133 | 0.199 | 0.307 | 0.484 | 0.829 | 1.254 | 1.911 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)