指标别名
IL 2; IL2; IL-2; interleukin 2; T cell growth factor; TCGF
免疫原
Expression system for standard: E.coli; Immunogen sequence: A21-Q155
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0399)
背景资料
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4.
试剂盒内容
| Catalog number | Description | Quantity |
| EK0399-CAP | 96-well plate precoated with anti- rat IL-2 antibody | 1 |
| EK0399-ST | Lyophilized recombinant rat IL-2 standard | 10ng/tubex2 |
| EK0399-DA | Biotinylated anti- rat IL-2 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 62 | 243 | 1041 | 56 | 254 | 1022 |
| Standard deviation | 4.71 | 15.06 | 53.09 | 4.81 | 17.52 | 71.54 |
| CV(%) | 7.6% | 6.2% | 5.1% | 8.6% | 6.9% | 7% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.053 | 0.116 | 0.171 | 0.286 | 0.478 | 0.792 | 1.467 | 2.454 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)