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Human CD25/IL2RA ELISA Kit

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EK0400

  • 96T ¥2280
  • 货期: 现货
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产品名称
Human CD25/IL2RA ELISA Kit
规格/价格
96T|2280
指标别名
CD25; IDDM10; IL 2 RA; IL 2 receptor subunit alpha; IL 2R subunit alpha; IL2 RA; IL2R; IL2RA; interleukin 2 receptor, alpha; p55; TAC antigen; TCGFR
免疫原
NSO,E22-C213
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名称
IL2RA
基因名全称
interleukin 2 receptor, alpha
Uniprot ID
P01589  
文献引用格式
Human CD25/IL2RA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0400)
背景资料
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0400-CAP96-well plate precoated with anti- human CD25 antibody1
EK0400-STLyophilized recombinant human CD25 standard10ng/tubex2
EK0400-DABiotinylated anti- human CD25 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)14071923531417852310
Standard deviation6.5841.794.127.6147.197.02
CV(%)4.75.845.464.2
典型数据示例
Concentration (pg/ml)031.262.512525050010002000
O.D.0.0420.1080.1580.260.4630.781.3572.098
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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