产品名称
Human CD25/IL2RA ELISA Kit
指标别名
CD25; IDDM10; IL 2 RA; IL 2 receptor subunit alpha; IL 2R subunit alpha; IL2 RA; IL2R; IL2RA; interleukin 2 receptor, alpha; p55; TAC antigen; TCGFR
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
interleukin 2 receptor, alpha
文献引用格式
Human CD25/IL2RA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0400)
背景资料
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0400-CAP | 96-well plate precoated with anti- human CD25 antibody | 1 |
| EK0400-ST | Lyophilized recombinant human CD25 standard | 10ng/tubex2 |
| EK0400-DA | Biotinylated anti- human CD25 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 140 | 719 | 2353 | 141 | 785 | 2310 |
| Standard deviation | 6.58 | 41.7 | 94.12 | 7.61 | 47.1 | 97.02 |
| CV(%) | 4.7 | 5.8 | 4 | 5.4 | 6 | 4.2 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.042 | 0.108 | 0.158 | 0.26 | 0.463 | 0.78 | 1.357 | 2.098 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)