产品名称
Mouse CD25/IL2RA ELISA Kit
指标别名
CD25; IDDM10; IL 2 RA; IL 2 receptor subunit alpha; IL 2R subunit alpha; IL2 RA; IL2R; IL2RA; interleukin 2 receptor, alpha; p55; TAC antigen; TCGFR
免疫原
Expression system for standard: NS0; Immunogen sequence: E22-K236
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Interleukin-2 receptor subunit alpha
文献引用格式
Mouse CD25/IL2RA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0915)
背景资料
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0915-CAP | 96-well plate precoated with anti- mouse CD25 antibody | 1 |
| EK0915-ST | Lyophilized recombinant mouse CD25 standard | 10ng/tube×2 |
| EK0915-DA | Biotinylated anti- mouse CD25 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 143 | 707 | 2735 | 152 | 647 | 2781 |
| Standard deviation | 7.29 | 51.61 | 142.22 | 9.72 | 49.81 | 194.67 |
| CV(%) | 5.1% | 7.3% | 5.2% | 6.4% | 7.7% | 7% |
典型数据示例
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.095 | 0.132 | 0.159 | 0.232 | 0.337 | 0.568 | 1.079 | 1.882 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)