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Mouse CD25/IL2RA ELISA Kit

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EK0915

  • 96T ¥2480
  • 货期: 现货
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产品名称
Mouse CD25/IL2RA ELISA Kit
规格/价格
96T|2480
指标别名
CD25; IDDM10; IL 2 RA; IL 2 receptor subunit alpha; IL 2R subunit alpha; IL2 RA; IL2R; IL2RA; interleukin 2 receptor, alpha; p55; TAC antigen; TCGFR
免疫原
Expression system for standard: NS0; Immunogen sequence: E22-K236
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
78pg/ml-5000pg/ml
基因名称
IL2RA
基因名全称
Interleukin-2 receptor subunit alpha
Uniprot ID
P01590  
文献引用格式
Mouse CD25/IL2RA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0915)
背景资料
CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
试剂盒内容
Catalog numberDescriptionQuantity
EK0915-CAP96-well plate precoated with anti- mouse CD25 antibody1
EK0915-STLyophilized recombinant mouse CD25 standard10ng/tube×2
EK0915-DABiotinylated anti- mouse CD25 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)14370727351526472781
Standard deviation7.2951.61142.229.7249.81194.67
CV(%)5.1%7.3%5.2%6.4%7.7%7%
典型数据示例
Concentration (pg/ml)078156312625125025005000
O.D.0.0950.1320.1590.2320.3370.5681.0791.882
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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