指标别名
Hematopoietic growth factor; IL 3; IL3; IL-3; Interleukin 3; Mast cell growth factor; MCGF; MULTI CSF; P cell stimulating factor
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human IL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0402)
背景资料
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0402-CAP | 96-well plate precoated with anti- human IL-3 antibody | 1 |
| EK0402-ST | Lyophilized recombinant human IL-3 standard | 10ng/tubex2 |
| EK0402-DA | Biotinylated anti- human IL-3 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 33 | 205 | 387 | 31 | 190 | 374 |
| Standard deviation | 1.68 | 9.63 | 27.86 | 1.73 | 8.93 | 28.42 |
| CV(%) | 5.1 | 4.7 | 7.2 | 5.6 | 4.7 | 7.6 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.027 | 0.073 | 0.108 | 0.195 | 0.353 | 0.674 | 1.188 | 2.014 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)