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Human IL3 ELISA Kit

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EK0402

  • 96T ¥1880
  • 货期: 现货
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产品名称
Human IL3 ELISA Kit
规格/价格
96T|1880
指标别名
Hematopoietic growth factor; IL 3; IL3; IL-3; Interleukin 3; Mast cell growth factor; MCGF; MULTI CSF; P cell stimulating factor
免疫原
E.coli,A20-F152
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
IL3
基因名全称
interleukin 3
Uniprot ID
P08700  
文献引用格式
Human IL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0402)
背景资料
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0402-CAP96-well plate precoated with anti- human IL-3 antibody1
EK0402-STLyophilized recombinant human IL-3 standard10ng/tubex2
EK0402-DABiotinylated anti- human IL-3 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3320538731190374
Standard deviation1.689.6327.861.738.9328.42
CV(%)5.14.77.25.64.77.6
典型数据示例
Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0270.0730.1080.1950.3530.6741.1882.014
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
实验时长
3.5h

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