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Rat IL3 ELISA Kit

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EK1324

  • 96T ¥2280
  • 货期: 现货
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产品名称
Rat IL3 ELISA Kit
规格/价格
96T|2280
指标别名
Hematopoietic growth factor; IL 3; IL3; IL-3; Interleukin 3; Mast cell growth factor; MCGF; MULTI CSF; P cell stimulating factor
免疫原
Expression system for standard: E.coli; Immunogen sequence: I27-C169
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8pg/ml-500pg/ml
基因名称
IL3
基因名全称
interleukin 3
Uniprot ID
P04823  
文献引用格式
Rat IL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1324)
背景资料
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1324-CAP96-well plate precoated with anti- rat IL-3 antibody1
EK1324-STLyophilized recombinant rat IL-3 standard10ng/tube×2
EK1324-DABiotinylated anti- rat IL-3 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)16912561484249
Standard deviation0.85.3616.640.775.3717.43
CV(%)5%5.9%6.5%5.5%6.4%7%
典型数据示例
Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0290.1380.2130.3650.651.0741.6732.331
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants and Serum

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