指标别名
Hematopoietic growth factor; IL 3; IL3; IL-3; Interleukin 3; Mast cell growth factor; MCGF; MULTI CSF; P cell stimulating factor
免疫原
Expression system for standard: E.coli; Immunogen sequence: I27-C169
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1324)
背景资料
The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1324-CAP | 96-well plate precoated with anti- rat IL-3 antibody | 1 |
| EK1324-ST | Lyophilized recombinant rat IL-3 standard | 10ng/tube×2 |
| EK1324-DA | Biotinylated anti- rat IL-3 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 16 | 91 | 256 | 14 | 84 | 249 |
| Standard deviation | 0.8 | 5.36 | 16.64 | 0.77 | 5.37 | 17.43 |
| CV(%) | 5% | 5.9% | 6.5% | 5.5% | 6.4% | 7% |
典型数据示例
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.029 | 0.138 | 0.213 | 0.365 | 0.65 | 1.074 | 1.673 | 2.331 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum