产品名称
Human IL-8/CXCL8 ELISA Kit
指标别名
C X C motif chemokine 8; CXCL8; CXCL8; IL8; CXCL8/IL-8; Emoctakin; GCP 1; GCP1; IL 8; IL 8(1 77); IL8; IL-8; IL8/NAP1 form I Interleukin 8; IL8/NAP1 form II; IL8/NAP1 form VI; interleukin 8; MDNCF; MDNCF b IL 8(5 77) IL 8(6 77); NAF; NAF IL 8(7 77); NAP 1; NAP1; Neutrophil activating factor; Protein 3 10C
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml (cellculturesupernates) 7.8 pg/ml - 500 pg/ml (serumplasma)
基因名全称
chemokine (C-X-C motif) ligand 8
文献引用格式
Human IL-8/CXCL8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0413)
背景资料
Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0413-CAP | 96-well plate precoated with anti- human IL-8 antibody | 1 |
| EK0413-ST | Lyophilized recombinant human IL-8 standard | 10ng/tubex2 |
| EK0413-DA | Biotinylated anti- human IL-8 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 16 | 66 | 234 | 16 | 63 | 230 |
| Standard deviation | 0.72 | 2.83 | 14.27 | 0.8 | 3.84 | 17.48 |
| CV(%) | 4.5 | 4.3 | 6.1 | 5 | 6.1 | 7.6 |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.003 | 0.170 | 0.321 | 0.594 | 1.121 | 1.878 | 2.552 | 2.779 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
联系我们
关注我们
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
