Human Leptin/LEP ELISA Kit

96T/2480

LEP; leptin; OB; Obese protein; Obesity factor; OBS

E.coli, V22-C167

There is no detectable cross-reactivity with other relevant proteins.

62.5 pg/ml - 4000 pg/ml

LEP

leptin

P41159  

AB_2800328

Human Leptin/LEP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0437)

Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

25-30分钟

Catalog number	Description	Quantity
EK0437-CAP	96-well plate precoated with anti- human Leptin antibody	1
EK0437-ST	Lyophilized recombinant human Leptin standard	10ng/tubex2
EK0437-DA	Biotinylated anti- human Leptin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	81	485	1057	75	444	996
Standard deviation	6.15	32.49	54.96	6.3	35.52	69.72
CV(%)	7.6	6.7	5.2	8.4	8	7

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.002	0.021	0.047	0.105	0.280	0.635	1.395	2.302

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h