Mouse Leptin/LEP ELISA Kit

96T/2480

LEP; leptin; OB; Obese protein; Obesity factor; OBS

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

LEP

Leptin

P41160  

Mouse Leptin/LEP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0438)

Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Mouse ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and mouse DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of mouse chromosome 7q. The standard product used in this kit is recombinant mouse Leptin.

15-20分钟

Catalog number	Description	Quantity
EK0438-CAP	96-well plate precoated with anti- mouse Leptin antibody	1
EK0438-ST	Lyophilized recombinant mouse Leptin standard	10ng/tubex2
EK0438-DA	Biotinylated anti- mouse Leptin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	104	827	1634	104	819	1564
Standard deviation	7.28	40.52	114.38	7.9	40.13	131.37
CV(%)	7%	4.9%	7%	7.6%	4.9%	8.4%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.104	0.172	0.213	0.241	0.411	0.923	1.135	1.714

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h