产品名称
Human OPG/TNFRSF11B ELISA Kit
指标别名
TNF receptor superfamily member 11b; OCIF; OPG; PDB5; TR1; TNFRSF11B
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
93.7 pg/ml - 6000 pg/ml
基因名全称
tumor necrosis factor receptor superfamily, member 11b
文献引用格式
Human OPG/TNFRSF11B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0480)
背景资料
Osteoprotegerin(OPG) is identical to osteoclastogenesis inhibitory factor(OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0480-CAP | 96-well plate precoated with anti- human OPG antibody | 1 |
EK0480-ST | Lyophilized recombinant human OPG standard | 10ng/tubex2 |
EK0480-DA | Biotinylated anti- human OPG antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 132 | 650 | 2696 | 130 | 692 | 2678 |
Standard deviation | 10.42 | 37.7 | 107.84 | 11.57 | 51.2 | 136.57 |
CV(%) | 7.9 | 5.8 | 4 | 8.9 | 7.4 | 5.1 |
典型数据示例
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.002 | 0.123 | 0.245 | 0.485 | 0.907 | 1.482 | 2.050 | 2.385 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva and Urine