产品名称
Mouse OPG/TNFRSF11B ELISA Kit
指标别名
TNF receptor superfamily member 11b; OCIF; OPG; PDB5; TR1; TNFRSF11B
免疫原
Expression system for standard: NS0; Immunogen sequence: E22-L401
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor receptor superfamily member 11B
文献引用格式
Mouse OPG/TNFRSF11B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0481)
背景资料
Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0481-CAP | Anti-Mouse Tnfrsf11b Pre-coated 96-well strip microplate | 1 |
EK0481-ST | Mouse Tnfrsf11b Standard | 10ng/tube×2 |
EK0481-DA | Mouse Tnfrsf11b Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 133 | 800 | 3535 | 139 | 767 | 3643 |
Standard deviation | 5.85 | 54.4 | 187.35 | 7.39 | 55.39 | 225.86 |
CV(%) | 4.4% | 6.8% | 5.3% | 4.6% | 7.3% | 6.2% |
典型数据示例
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.000 | 0.082 | 0.173 | 0.294 | 0.522 | 0.976 | 1.585 | 2.018 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA)