Human PDGF-AB ELISA Kit

96T/2280

PDGF A chain; PDGF B chain; PDGF1; PDGF2; PDGFA; PDGFB; Platelet derived growth factor AB heterodimer; PDGFB; PDGFA

E.coli, (S87-T211)+(S82-T190)

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2000 pg/ml

PDGFA/PDGFB

platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide

P01127/P04085  

Human PDGF-AB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0484)

The platelet-derived growth factor(PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly(96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus(SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog(PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.

25-30分钟

Catalog number	Description	Quantity
EK0484-CAP	96-well plate precoated with anti- human PDGF-AB antibody	1
EK0484-ST	Lyophilized recombinant human PDGF-AB standard	10ng/tubex2
EK0484-DA	Biotinylated anti- human PDGF-AB antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	60	259	808	62	271	865
Standard deviation	3.96	14.76	51.71	4.52	19.51	68.33
CV(%)	6.6	5.7	6.4	7.3	7.2	7.9

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.023	0.126	0.252	0.423	0.922	1.358	1.856	2.195

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h