Rat PDGF-AB ELISA Kit

96T/2280

PDGF A chain; PDGF B chain; PDGF1; PDGF2; PDGFA; PDGFB; Platelet derived growth factor AB heterodimer; PDGFB; PDGFA

Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182)

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

Pdgfa/Pdgfb

platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide

G3V882/P28576  

Rat PDGF-AB ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0485)

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

15-20分钟

Catalog number	Description	Quantity-CAP	96-well plate precoated with anti- rat PDGF-AB antibody	1-ST	Lyophilized recombinant rat PDGF-AB standard	10ng/tubex2-DA	Biotinylated anti- rat PDGF-AB antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	86	282	885	83	277	930
Standard deviation	4.9	14.38	58.41	4.98	16.89	74.4
CV(%)	5.7%	5.1%	6.6%	6%	6.1%	8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.046	0.178	0.288	0.519	0.820	1.335	1.989	2.426

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)