产品名称
Human P-Selectin/SELP ELISA Kit
指标别名
CD62; CD62P; GMP 140; GMP140; GMRP; Granule membrane protein 140; GRMP; LECAM3; P selectin; PADGEM; PSEL; P-selectin; SELP
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
selectin P (granule membrane protein 140kDa, antigen CD62)
文献引用格式
Human P-Selectin/SELP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0505)
背景资料
P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0505-CAP | 96-well plate precoated with anti- human sP-Selectin antibody | 1 |
| EK0505-ST | Lyophilized recombinant human sP-Selectin standard | 10ng/tubex2 |
| EK0505-DA | Biotinylated anti- human sP-Selectin antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 322 | 1820 | 5020 | 331 | 1849 | 5389 |
| Standard deviation | 15.13 | 121.94 | 256.02 | 21.18 | 144.22 | 301.78 |
| CV(%) | 4.7 | 6.7 | 5.1 | 6.4 | 7.8 | 5.6 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.014 | 0.105 | 0.17 | 0.326 | 0.596 | 0.979 | 1.507 | 1.967 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)