Mouse P-Selectin/SELP ELISA Kit

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EK0506

96T ￥2280
货期：现货

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产品名称

Mouse P-Selectin/SELP ELISA Kit

规格/价格

96T|2280

指标别名

CD62; CD62P; GMP 140; GMP140; GMRP; Granule membrane protein 140; GRMP; LECAM3; P selectin; PADGEM; PSEL; P-selectin; SELP

免疫原

Expression system for standard: NS0; Immunogen sequence: W42-A709

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

312pg/ml-20000pg/ml

基因名称

SELP

基因名全称

P-selectin

Uniprot ID

Q01102

文献引用格式

Mouse P-Selectin/SELP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0506)

背景资料

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

试剂盒内容

Catalog number	Description	Quantity
EK0506-CAP	Anti-Mouse Selp Pre-coated 96-well strip microplate	1
EK0506-ST	Mouse Selp Standard	20ng/tube×2
EK0506-DA	Mouse Selp Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	596	2016	7521	640	1834	7467
Standard deviation	28.6	106.84	443.73	32	105	500.28
CV(%)	4.8%	5.3%	5.9%	5%	5.9%	6.7%

典型数据示例

Concentration (ng/ml)	0	0.312	0.62	1.25	2.5	5	10	20
O.D.	0.025	0.195	0.311	0.514	0.801	1.312	1.763	2.029

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Mouse P-Selectin/SELP ELISA Kit

EK0506

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(8) All Species Mouse(8) 清除

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