产品名称
Human VEGFR1/FLT1 ELISA Kit
指标别名
FLT; FLT 1; FLT1; FLT-1; Fms like tyrosine kinase 1; FRT; sflt1; Tyrosine protein kinase FRT; VEGF receptor 1; VEGFR 1; VEGFR1; VEGFR-1; VEGFR-1/FLT-1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
fms-related tyrosine kinase 1
文献引用格式
Human VEGFR1/FLT1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0543)
背景资料
sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0543-CAP | 96-well plate precoated with anti- human sVEGFR1 antibody | 1 |
EK0543-ST | Lyophilized recombinant human sVEGFR1 standard | 10ng/tubex2 |
EK0543-DA | Biotinylated anti- human sVEGFR1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 237 | 1369 | 4420 | 240 | 1352 | 4316 |
Standard deviation | 9.71 | 106.78 | 203.32 | 11.28 | 131.14 | 202.85 |
CV(%) | 4.1 | 7.8 | 4.6 | 4.7 | 9.7 | 4.7 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.059 | 0.104 | 0.151 | 0.243 | 0.412 | 0.771 | 1.503 | 2.442 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA)