产品名称
Mouse VEGFR1/FLT1 ELISA Kit
指标别名
FLT; FLT 1; FLT1; FLT-1; Fms like tyrosine kinase 1; FRT; sflt1; Tyrosine protein kinase FRT; VEGF receptor 1; VEGFR 1; VEGFR1; VEGFR-1; VEGFR-1/FLT-1
免疫原
Expression system for standard: NS0; Immunogen sequence: Y23-E759
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Vascular endothelial growth factor receptor 1
文献引用格式
Mouse VEGFR1/FLT1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0589)
背景资料
Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0589-CAP | 96-well plate precoated with anti- mouse VEGFR1 antibody | 1 |
EK0589-ST | Lyophilized recombinant mouse VEGFR1 standard | 10ng/tube×2 |
EK0589-DA | Biotinylated anti- mouse VEGFR1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 210 | 954 | 6138 | 191 | 959 | 6693 |
Standard deviation | 8.82 | 66.78 | 349.86 | 11.26 | 74.8 | 468.51 |
CV(%) | 4.2% | 7% | 5.7% | 5.9% | 7.8% | 7% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.016 | 0.070 | 0.122 | 0.234 | 0.450 | 0.909 | 1.536 | 2.249 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum