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Human Survivin/BIRC5 ELISA Kit

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EK0641

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human Survivin/BIRC5 ELISA Kit
规格/价格
96T/2480
指标别名
API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; EPR 1; IAP4; SURVIVIN
免疫原
E.coli, M1-D142
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名称
BIRC5
基因名全称
baculoviral IAP repeat containing 5
Uniprot ID
O15392  
文献引用格式
Human Survivin/BIRC5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0641)
背景资料
Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0641-CAP96-well plate precoated with anti- human Survivin antibody1
EK0641-STLyophilized recombinant human Survivin standard4ng/tubex2
EK0641-DABiotinylated anti- human Survivin antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)20766012591926901195
Standard deviation15.5248.1851.6115.9357.2766.92
CV(%)7.57.34.18.38.35.6
典型数据示例
Concentration(pg/ml)062.5125250500100020004000
O.D.0.0300.0790.1290.2010.3790.7011.2581.968
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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