产品名称
Human Survivin/BIRC5 ELISA Kit
指标别名
API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; EPR 1; IAP4; SURVIVIN
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
baculoviral IAP repeat containing 5
文献引用格式
Human Survivin/BIRC5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0641)
背景资料
Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0641-CAP | 96-well plate precoated with anti- human Survivin antibody | 1 |
EK0641-ST | Lyophilized recombinant human Survivin standard | 4ng/tubex2 |
EK0641-DA | Biotinylated anti- human Survivin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 207 | 660 | 1259 | 192 | 690 | 1195 |
Standard deviation | 15.52 | 48.18 | 51.61 | 15.93 | 57.27 | 66.92 |
CV(%) | 7.5 | 7.3 | 4.1 | 8.3 | 8.3 | 5.6 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.030 | 0.079 | 0.129 | 0.201 | 0.379 | 0.701 | 1.258 | 1.968 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)