产品名称
Human CD127/IL7R ELISA Kit
指标别名
CD127; CDW127; IL 7 receptor subunit alpha; IL 7R alpha; IL 7R subunit alpha; IL 7RA; IL7R; IL-7R; IL 7R; IL7RA; ILRA; interleukin 7 receptor
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
interleukin 7 receptor
文献引用格式
Human CD127/IL7R ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0713)
背景资料
The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0713-CAP | 96-well plate precoated with anti- human IL7R antibody | 1 |
| EK0713-ST | Lyophilized recombinant human IL7R standard | 20ng/tube×2 |
| EK0713-DA | Biotinylated anti- human IL7R antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 410 | 2617 | 9061 | 443 | 2807 | 9233 |
| Standard deviation | 25.42 | 120.38 | 516.47 | 33.22 | 140.35 | 627.84 |
| CV(%) | 6.2 | 4.6 | 5.7 | 7.5 | 5 | 6.8 |
典型数据示例
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.032 | 0.059 | 0.098 | 0.167 | 0.274 | 0.59 | 1.061 | 1.929 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum